Stage Top Incubator
Offers precision temperature, humidity and CO2 control for cell culture on a microscope.
Enables to conduct short and long term (more than 2 weeks) Time-Lapse Imaging.
By applying Real-time Sample Feedback Regulation,
it can maintain an optimal cell culture environment
which is as same as the conventional CO2 incubator.
With our variety of options, we can offer solutions for abundant applications,
and leading the experiment to success.
Accurate and uniform temperature control
TOKAI HIT Heating Quality
Tokai Hit's original Top Heater is proven to distribute heat uniformly within the Chamber
regardless of the type of vessels.
No interference by objective
With unique Top Heater Heating Time regulation, the bottom of Chamber is access-free for variety of objectives. (No metal plate at the bottom.)
Real-time Sample Feedback Regulation
Sterilized temperature sensor and magnetic lids make it easy to measure
the temp. of culture media upon research needs.
The controller regulates the heater based on the sensor signal to keep sample at the target temp. accurately.
Apply Internal Humidifier to keep high humidity condition during the imaging
Keeps high humidity inside the chamber by heating the distilled water in the Bath Unit.
Internal Humidifier minimizes the change in concentration of media by keeping the humidity inside the chamber.
Stable CO2 environment
The controller mixes 100%CO2 gas and the surrounding air automatically. Stable gas concentration inside the Chamber is obtained by keep sending the mixed gas to the Chamber.
(※In case of controller with a built-in digital gas mixer)
Stress-Free Quality
Prevent the focus drift
In addition to PID control, Continuous Current Control minimizes the focus drift generated by thermal expansion and it also prevents light intensity change compared to the conventional ON/OFF control.
STX-APP (Software)
Simple operation of GUI will assist to visualize the preparation to setting and lead your cell culture to success.
Programmable Control
The system includes the software to program temp. and CO2/O2 concentration
as this function allows to expand the variety of experiment.
Screen Capture
Captures the PC screen to transfer images to smart-phones and tablets.
Enables to see the image at home. ＊PC must be connected with internet.
Data Logging
Logs the temperature of each heaters, sample temperature and gas concentration
and saves the data in CSV format.
Line-up
（専用ピエゾステージ対応）For Nikon Ti/Ti2 exclusive piezo stage适用于Nikon Ti/Ti2 Piezo载物台
（専用ピエゾステージ対応）For Nikon Ti/Ti2 exclusive piezo stage适用于Nikon Ti/Ti2 Piezo载物台
（Laser Safety Hood対応）For Leica manual/motorized stage
（with Laser Safety Hood）适用于Leica 手动/电动载物台
（附带聚光器盖）
Super Z Galvo 载物台专用
MCL Nano-Z500用For ASI PZ-2000,
MCL Nano-Z500适用于ASI PZ-2000、
及MCL Nano-Z500载物台
* Cooling/Heating Chamber适用于各种手动/电动载物台
Specification
| STXG with built-in gas mixer |
STXF with analog flowmeter |
|||
|---|---|---|---|---|
| Temperature | Temperature control | PID control | ||
| Increments | 0.1℃ by digital switches | |||
| Setting method | Touch Panel or PC | |||
| Possible temp. setting range* | SAMPLE TEMP | 30℃～40℃ | ||
| TOP HEATER | 10℃～65℃ | |||
| BATH HEATER | 10℃～50℃ | |||
| STAGE HEATER | 10℃～50℃ | |||
| LENS HEATER | 10℃～45℃ | |||
| Rise time | 10 minutes to reach 50℃ | |||
| Accuracy | Within ±0.3℃ at each heater surface（in our terms and conditions） | |||
| Sensor | Pt 100 | |||
| Humidity | Humidification control | Forced humidification by heating Bath Unit | ||
| Condensation prevention | Top Heater equipped with glass heater | |||
| Gas | CO2 concentration range | 5％～20％ | ー | |
| Control method | PID control | Precision needle valve | ||
| Accuracy | Within ±0.1％ | Within±2％ FS | ||
| Gas type | 100% CO2 | 5%CO2 | ||
| Input gas pressure | 0.1Mpa～0.15MPa | |||
| Output gas pressure | 160 ml/min | 75 ml/min～ 250 ml/min |
||
| Power source | 100 V～240 V AC ±10% 50/60Hz | |||
| Maximum power consumption | 110W | 90W | ||
Add-on options
Program fluidic control system
PMD-D35
Perfusion, Media Exchange, Drug Delivery and Mixing can be easily programmed and done without disturbing your sample.
You can use the commercially available 35 mm dish.
| Specification |
|
|---|---|
| Components |
|
Perfusion/Media exchange system
KSX-Type1
Perfusion/Media exchange without removing a dish lid is possible.
Prevents media evaporation and contamination during long-term imaging.
| Specification |
|
|---|---|
| Components |
|
One-push drug delivery system
KSX-Type2
Rapid and vibration-free drug delivery is possible.
Prevents media evaporation and contamination during long-term imaging.
| Specification |
|
|---|
Digital Gas Mixer
Digital Gas Mixer for Stage Top Incubator. You can choose depending on the usage gas cylinder.
For STX series
STX-CO2O2
Hypoxia・For low oxygen
Creates hypoxia and normal conditions with easy settings.
| O2 concentration | 0.1～18.0％ |
|---|---|
| CO2 concentration | 5.0～20.0％ |
| Gas cylinder | 100％CO2 & 100%N2 |
| Dimention | W160 × D271 × H250 mm |
STX-CO2
For CO2 concentration
*For STXF Controller
concentration: 5.0％～20.0％ CO2 flow rate: 160ml/min
| CO2 concentration | 5.0～20.0％ |
|---|---|
| Gas cylinder | 100％CO2 |
| Dimention | W115×D271×H250 |
STX-O2
For O2 concentration
＊Must use with STX-CO2
| O2 concentration | 0.1～18.0％ |
|---|---|
| Gas cylinder | 100%N2 |
| Dimention | W115×D271×H250 |
Stand alone model
GM-8000
Hypoxia・For low oxygen
Creates hypoxia and normal conditions with easy settings.
| O2 concentration | 0.1～18.0％ |
|---|---|
| CO2 concentration | 5.0～20.0％ |
| Gas cylinder | 100％CO2 & 100%N2 |
| Dimention | W175×D160×H280 (mm) |
GM-3000
For CO2 conc.& flow rate
Adjustable CO2 concentration and flow rate. CO2 concentration: 1.0 – 20.0% Flow rate: 50ml/min – 200ml/min
| CO2 concentration | 1.0～20.0％ |
|---|---|
| CO2 flow rate | 50～200 ml/min |
| Gas cylinder | 100％CO2 |
| Dimention | W121×D174×H157 (mm) |
Mini CO2 regulator (only available in the US and Japan)
MG1
There is no need to prepare a large gas cylinder and no regulator operation is required.
The gas is supplied at the optimal flow rate for the Tokai Hit incubator with one push.
| Specification |
|
|---|---|
| Exclusive gas cartridge |
|
External Humidifier
TPiDE-HUMID
By adding an external humidifier to the Tokai Hit incubation system,
it is possible to perform long-term culture for 3 to 4 days without refilling the water to the chamber during imaging.
| Components |
|
|---|
Accessories
Stage Adapter
For WSKMX / IX3WX chamber
| Model | MK-IX3 |  |
|---|---|---|
| Compatible stage | Evident IX3-SSU, IX3-SVR | |
| Model | MK-SIG |  |
| Compatible stage | Evident BX3-SSU, IX2-SFR, IX2-SVL2 | |
| Model | TI2-ZILCS |  |
| Compatible stage | Nikon TI2-S-SE-E, TI2-S-SS-E | |
| Model | TI2-RA |  |
| Compatible stage | Nikon TC-S-SR, TC-S-SRF | |
| Model | TID-ZILCS |  |
| Compatible stage | Nikon TI-S-E, TI-S-ER | |
| Model | MK-RA |  |
| Compatible stage | Nikon Ti-SR, Ti-SSR |
For TIZWX, TIZBX chamber
| Model | TI2-NA |  |
|---|---|---|
| Compatible stage | Nikon TI2-S-SE-E, TI2-S-SS-E | |
| Model | TID-NA |  |
| Compatible stage | Nikon TI-S-E, TI-S-ER |
For WELSX chamber
| Model | WELSX-K |  |
|---|---|---|
| Compatible stage | For XY motorized stage（160×110mm opening） | |
| Model | WELSX-RA |  |
| Compatible stage | Nikon TI-S-E, TI-S-ER | |
| Model | WELSX-TIM |  |
| Compatible stage | TI-S-E, TI-S-ER | |
| Model | WELSX-TIP |  |
| Compatible stage | Nikon Ti/Ti2 piezo stage | |
| Model | WELSX-IX3 |  |
| Compatible stage | Evident IX3-SSU, IX3-SVR | |
| Model | WELSX-SIG |  |
| Compatible stage | Evident BX3-SSU, IX2-SFR, IX2-SVL2 |
For GSI2X chamber
| Model | GSI2X-K |  |
|---|---|---|
| Compatible stage | For XY motorized stage （160×110mm opening） |
Dish Attachment
| Model | UNIV2-D35-2 |  |
|---|---|---|
| For 35mm dish ×2 | ||
| Model | UNIV2-D35-3 |  |
| For 35mm dish ×3 | ||
| Model | UNIV2-D35-4 |  |
| For 35mm dish ×4 | ||
| Model | UNIV2-D35-5 | |
| For 35mm dish ×5 | ||
| Model | UNIV2-D35-6 |  |
| For 35mm dish ×6 |
Reusable 35mm dish
*Cyto-cell Chamber (Auto-clavable)
＜Collaborative development with Prof. Takafumi Inoue, Waseda Univ.＞
Features
|    |
For a small amount of medium
|  |
For wide range observation
|  |
Digital Thermometer for research
By using the fine probe, it minimizes the heat sink of the object to the sensor, and precise temperature measurement is possible.
| Model：MC1000 Indicate temp. by 1 degree C/0.1 degree C units K-type thermocouple ＜Components＞ ・Digital Thermometer ・Thermo Probe (TSU-200F) |  |
| Model | TSU-200F |  |
|---|---|---|
| Thermo Prove (Sensor type) | ||
| Model | HD1500 |  |
| Extension Wire (1.5m) |
IN/OUT Pipe for Media Exchange/Drug Delivery
For media exchange and drug delivery with incubation system for upright microscopes etc..
|  |
35mm Dish Spacer
When using the 35mm dish from IWAKI, Greiner and Nunc, recommended to use Dish Spacer at the bottom of the dish.
|  |
Customization
We are accepting customization according to the application and conditions.
Please feel free to contact us.
Abundant experiences more than 100 products per year
Our experienced staff and engineers will accomodate your requests.
Customization reference 1
Incubation system for MED64
This device has been designed on the assumptions of an experiment of electrophysiology.
Enable the low noise attribution under the cell culturing environment.
With built-in digital gas mixer
Model：INUG2M-MED
With built-in analog flow meter
Model：INUM-MED-F1
Temperature Controller only
Model：INUM-MED
Customization reference 2
Chamber for MEMS
Customized attachments or spacers are available for your usage vessels.
In addition, attachments for patch clamp are also available.
Customization reference 3
Built-in Heater
It is possible to customize heater for your system.
We can manage the length of cable, corresponding temperature etc..
クリーニング
Olympus IX83
Nikon Ti2
Leica DMi8
ZEISS Axio Observer
STX-APP
Cleaning method
For Olympus IX83
For Nikon Ti2
Leica DMi8
For ZEISS Axio Observer
STX-APP
活细胞培养系统安装方法 For奥林巴斯显微镜IX83
活细胞培养系统安装方法 For尼康Ti2
活细胞培养系统安装方法 For徕卡DMi8
活细胞培养系统安装方法 For蔡司Axio Observer
活细胞培养系统的清洁方法
STX-APP软件安装和使用方法
活细胞培养系统的温度传感器设置方法
程序控制流体系统PMD-D35介绍视频
显微镜用恒温箱的安装方法 For徕卡DMi8
活细胞培养系统的各种容器适配器的安装方法
Human RPE1 cells stably expressing H2B-EGFP
画像提供：Aussie Suzuki, PI, Suzuki Lab, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison
Hela DIC 3days 9panel montage
画像提供：Dr. Simon Watkins, University of Pittsburgh Department of Cell Biology
Hela DIC 3days
画像提供：University of Pittsburgh Department of Cell Biology
live mouse embryonic fibroblast cell
画像提供：Moerner Lab, Stanford University
Human Bone Cancer Cells
画像提供：Lauren Piedmont Alvarenga of the Nikon Imaging Center @ Harvard Medical School
HeLa, HEK293
画像提供：Tomomi Nemoto Ph.D, National Institute for Physiological Sciences, Kentaro koboyashi Ph.D., Nikon Imaging Center @ Hokkaido University,
COS7
画像提供：Tomomi Nemoto Ph.D, National Institute for Physiological Sciences, Kentaro koboyashi Ph.D., Nikon Imaging Center @ Hokkaido University,
Cell ORIGAMI
画像提供：Dr. Kaori Shigetomi(Kuribayashi), Hokkaido University, Shoji Takeuchi, Ph.D., Institute of Industrial Science, The University of Tokyo
Cell cycle progressions in HeLa cells
画像提供：N.Komatsu, A.Sakaue-Sawano and A.Miyawaki, RIKEN Center for Brain Science
Multiphoton imaging of pancreatic islets
画像提供：Dr. Simon Watkins, Center for Biologic Imaging, University of Pittsburgh
Multiphoton imaging of pancreatic islets_Color Combine
画像提供：Dr. Simon Watkins, Center for Biologic Imaging, University of Pittsburgh
Wound healing in epithelial cells, 96 hour time-lapse
画像提供：Dr. Simon Watkins and Dr. Mingzhu Jin, Center for Biologic Imaging, University of Pittsburgh
Super-resolution (STED) imaging of mitochondria and ER in fibroblasts
画像提供：Dr. Simon Watkins and Dr. Mike Calderon, Center for Biologic Imaging, University of Pittsburgh
HeLa cell ― Cell cycle indicator Fucci
画像提供：Tetsushi Hoshida, Asako Sakaue, Atsushi Miyawaki, RIKEN
Human RPE1 cells stably expressing H2B-EGFP
Courtesy of Aussie Suzuki, PI, Suzuki Lab, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison
Hela DIC 3days 9panel montage
Courtesy of Dr. Simon Watkins, University of Pittsburgh Department of Cell Biology
Hela DIC 3days
Courtesy of University of Pittsburgh Department of Cell Biology
live mouse embryonic fibroblast cell
Courtesy of Moerner Lab, Stanford University
Human Bone Cancer Cells
Courtesy of Lauren Piedmont Alvarenga of the Nikon Imaging Center @ Harvard Medical School
HeLa, HEK293
Courtesy of Tomomi Nemoto Ph.D, National Institute for Physiological Sciences, Kentaro koboyashi Ph.D., Nikon Imaging Center @ Hokkaido University,
COS7
Courtesy of Tomomi Nemoto Ph.D, National Institute for Physiological Sciences, Kentaro koboyashi Ph.D., Nikon Imaging Center @ Hokkaido University,
Cell ORIGAMI
Courtesy of Dr. Kaori Shigetomi(Kuribayashi), Hokkaido University, Shoji Takeuchi, Ph.D., Institute of Industrial Science, The University of Tokyo
Cell cycle progressions in HeLa cells
Courtesy of N.Komatsu, A.Sakaue-Sawano and A.Miyawaki, RIKEN Center for Brain Science
Multiphoton imaging of pancreatic islets
Courtesy of Dr. Simon Watkins, Center for Biologic Imaging, University of Pittsburgh
Multiphoton imaging of pancreatic islets_Color Combine
Courtesy of Dr. Simon Watkins, Center for Biologic Imaging, University of Pittsburgh
Wound healing in epithelial cells, 96 hour time-lapse
Courtesy of Dr. Simon Watkins and Dr. Mingzhu Jin, Center for Biologic Imaging, University of Pittsburgh
Super-resolution (STED) imaging of mitochondria and ER in fibroblasts
Courtesy of Dr. Simon Watkins and Dr. Mike Calderon, Center for Biologic Imaging, University of Pittsburgh
HeLa cell ― Cell cycle indicator Fucci
Courtesy of Tetsushi Hoshida, Asako Sakaue, Atsushi Miyawaki, RIKEN
Hela DIC 3days 9panel montage
视频提供：Dr. Simon Watkins, University of Pittsburgh Department of Cell Biology
Hela DIC 3days
视频提供：University of Pittsburgh Department of Cell Biology
live mouse embryonic fibroblast cell
视频提供：Moerner Lab, Stanford University
Human Bone Cancer Cells
视频提供：Lauren Piedmont Alvarenga of the Nikon Imaging Center @ Harvard Medical School
HeLa, HEK293
视频提供：Tomomi Nemoto Ph.D, National Institute for Physiological Sciences, Kentaro koboyashi Ph.D., Nikon Imaging Center @ Hokkaido University,
COS7
视频提供：Tomomi Nemoto Ph.D, National Institute for Physiological Sciences, Kentaro koboyashi Ph.D., Nikon Imaging Center @ Hokkaido University,
Cell ORIGAMI
视频提供：Dr. Kaori Shigetomi(Kuribayashi), Hokkaido University, Shoji Takeuchi, Ph.D., Institute of Industrial Science, The University of Tokyo
Cell cycle progressions in HeLa cells
视频提供：N.Komatsu, A.Sakaue-Sawano and A.Miyawaki, RIKEN Center for Brain Science
Multiphoton imaging of pancreatic islets
视频提供：Dr. Simon Watkins, Center for Biologic Imaging, University of Pittsburgh
Multiphoton imaging of pancreatic islets_Color Combine
视频提供：Dr. Simon Watkins, Center for Biologic Imaging, University of Pittsburgh
Wound healing in epithelial cells, 96 hour time-lapse
视频提供：Dr. Simon Watkins and Dr. Mingzhu Jin, Center for Biologic Imaging, University of Pittsburgh
Super-resolution (STED) imaging of mitochondria and ER in fibroblasts
视频提供：Dr. Simon Watkins and Dr. Mike Calderon, Center for Biologic Imaging, University of Pittsburgh
HeLa cell ― Cell cycle indicator Fucci
视频提供：Tetsushi Hoshida, Asako Sakaue, Atsushi Miyawaki, RIKEN